Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays

doi: 10.1007/978-1-4939-6584-7_11

Figure Lengend Snippet: Overview of SMM strategy to identify RNA-binding small molecules. Small molecule libraries prepared in 384-well plates are utilized to print onto isocyanate-functionalized slides by the microarrayer. Prepared SMMs are next incubated with the labeled RNA of interest and scanned to detect hits

Article Snippet: Printing of Small Molecule Microarrays MicroGrid II Microarrayer (BioRobotics) or equivalent.

Techniques: RNA Binding Assay, Incubation, Labeling

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: The Z’ factors and the CV values of the HCI assays performed on the microarray chip platform.

Article Snippet: Three-dimensional (3D) bioprinting with a microarray spotter For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Microarray

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: Representative images from 72 h-incubated micropillar chips with 3D-cultured Hep3B cells, exposed to menadione (0.7 – 177 μM) for 48 h, and stained with the four fluorescent dyes: (A) TMRM for mitochondrial impairment, (B) Hoechst 33342 for DNA damage, (C) mBCl for changes in intracellular glutathione level, and (D) calcein AM for cell membrane integrity. The dose-dependent effect of menadione for all four assays was clearly observed from the microarray images obtained.

Article Snippet: Three-dimensional (3D) bioprinting with a microarray spotter For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Incubation, Cell Culture, Staining, Membrane, Microarray